Over the last decade there has been a growing interest in low-calorie alternatives to carbohydrate-based sweeteners. Recent publications have shown a dramatic increase in attention toward natural extracts such as the Stevia rebaudiana plant, not only for its sweetening effect but also for additional health benefits attributed to the plant. The major sweetening components are stevioside, rebaudioside A, rebaudioside C, and dulcoside A, each of which is over 300 times sweeter than sucrose based sweeteners. Because of this they are widely used in beverages and foodstuffs.

Choline is a water soluble essential nutrient that is produced in the body in small quantities. Choline is a precursor of acetylcholine which is an important neurotransmitter involved in muscle control and memory. Choline is a small, highly polar quaternary amine and will not be retained using a C18 column. The zwitterionic nature of Syncronis HILIC makes it ideal for the retention of this class of compound. This application note demonstrates the successful analysis of acetylcholine and choline using a Syncronis HILIC 1.7 µm column.

The Thermo Scientific Dionex UltiMate 3000 system is applied for the determination of metformin. The separation is performed on a Thermo Scientific Syncronis HILIC 5 µm column, with UV detection at 225 nm and a total run time of 6 minutes.

The Thermo Scientific Dionex UltiMate 3000 RSLC system is applied for the analysis of edrophonium, neostigmine, and pyridostigmine in plasma. The samples were prepared using Thermo Scientific SOLA WCX solid phase extraction (SPE) cartridges and the separation performed on a Thermo Scientific Syncronis HILIC HPLC column, with MS/MS detection. For Research Use Only. Not for use in diagnostic procedures.

A liquid chromatography method for the analysis of Biopterin, Dihydro-biopterin and Tetrahydro-biopterin was developed on a Thermo Scientific Syncronis HILIC HPLC column for a fast separation with a cycle time of 3 minutes while maintaining excellent peak shape and resolution.