We present a multi-residue instrumental method that can be applied for high-throughput screening and semi-quantitation of pesticide residues in food matrices at or below the current legislative requirements. A high-resolution, accurate-mass mass spectrometer operated in Full Scan – Variable Data-Independent Analysis (FS-vDIA)* mode provided an option for full spectrum filtering, retrospective analysis, and multi-parameter-based compound identification. The method was validated for 328 target pesticides, with an option for the future extension to a larger number. (*vDIA not available in US.)

Clinical research labs commonly use selective and cost-efficient LC-MS techniques for the analysis of immunosupressants drugs. Usually, the quantitative method is developed on a triple quadrupole mass spectrometer. Here, we evaluated a high-efficiency method implemented on a Q Exactive Focus hybrid quadrupole Orbitrap mass spectrometer for improved selectivity when compared to the conventional solution.

Forensic toxicologists need an economical solution to screen for a virtually unlimited number of compounds in urine. Here we present a method for screening using a dilute-and-shoot approach with the Q Exactive Focus mass spectrometer and Thermo Scientific ToxFinder software. For forensic use only.

Clinical research laboratories perform analysis of methylmalonic acid (MMA) using LC-MS because of that technique’s sensitivity and selectivity. Usually, the quantitative method is developed on a triple quadrupole mass spectrometer. Here we evaluated a method implemented on a Q Exactive Focus ultra-high resolution quadrupole-Orbitrap mass spectrometer for the best selectivity.

Chromatographic separation is required for simultaneous analysis of 25-hydroxyvitamin D3 (25OHD3) and its epimeric form 3-epi-25-hydroxyvitamin D3 (epi-25OHD3) in a liquid chromatography-mass spectrometry (LC-MS) method because both compounds have the same molecular formulas and the same fragmentation spectra. We developed an LC method that provides baseline separation between the two isobaric compounds. We coupled this chromatographic method to an MS method, which collects high-resolution MS/MS spectra for compound identification and quantitation.