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Rapid Analysis of 25-Hydroxyvitamin D3, epi-25-Hydroxyvitamin D3, and 25-Hydroxyvitamin D2 in Plasma for Clinical Research Using High-Resolution Mass Spectrometry Be the first to rate this application

rapid analysis 25hydroxyvitamin d3 epi25hydroxyvitamin d3 25hydroxyvitamin d2 plasma for clinical research using highresolution mass spectrometry

Description

Chromatographic separation is required for simultaneous analysis of 25-hydroxyvitamin D3 (25OHD3) and its epimeric form 3-epi-25-hydroxyvitamin D3 (epi-25OHD3) in a liquid chromatography-mass spectrometry (LC-MS) method because both compounds have the same molecular formulas and the same fragmentation spectra. We developed an LC method that provides baseline separation between the two isobaric compounds. We coupled this chromatographic method to an MS method, which collects high-resolution MS/MS spectra for compound identification and quantitation.
Market: Clinical Research
Keywords: Hypersil GOLD, SOLAμ, SPE, UltiMate 3000, Clinical Research, Micro-elution, Quantitation, Vitamin D, Q Exactive Focus
Matrix: Plasma
Author: Mindy Gao, James Goldberg, Thomas Carrell, and Marta Kozak
Affiliation: Thermo Fisher Scientific
Uploaded on 10/21/2016.

For Research Use Only. Not for use in diagnostic procedures.