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Rapid Analysis of 25-Hydroxyvitamin D3, epi-25-Hydroxyvitamin D3, and 25-Hydroxyvitamin D2 in Plasma for Clinical Research Using High-Resolution Mass Spectrometry
Description
Chromatographic separation is required for simultaneous analysis of 25-hydroxyvitamin D3 (25OHD3) and its epimeric form 3-epi-25-hydroxyvitamin D3 (epi-25OHD3) in a liquid chromatography-mass spectrometry (LC-MS) method because both compounds have the same molecular formulas and the same fragmentation spectra. We developed an LC method that provides baseline separation between the two isobaric compounds. We coupled this chromatographic method to an MS method, which collects high-resolution MS/MS spectra for compound identification and quantitation.| Market: | Clinical Research |
| Keywords: | Hypersil GOLD, SOLAμ, SPE, UltiMate 3000, Clinical Research, Micro-elution, Quantitation, Vitamin D, Q Exactive Focus |
| Matrix: | Plasma |
| Author: | Mindy Gao, James Goldberg, Thomas Carrell, and Marta Kozak |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
