Rapid Analysis of 25-Hydroxyvitamin D3, epi-25-Hydroxyvitamin D3, and 25-Hydroxyvitamin D2 in Plasma for Clinical Research Using High-Resolution Mass Spectrometry

Instrument Type: LCMSMS

Chromatographic separation is required for simultaneous analysis of 25-hydroxyvitamin D3 (25OHD3) and its epimeric form 3-epi-25-hydroxyvitamin D3 (epi-25OHD3) in a liquid chromatography-mass spectrometry (LC-MS) method because both compounds have the same molecular formulas and the same fragmentation spectra. We developed an LC method that provides baseline separation between the two isobaric compounds. We coupled this chromatographic method to an MS method, which collects high-resolution MS/MS spectra for compound identification and quantitation.

Fast and reliable method for the analysis of methylmalonic acid from human plasma

Instrument Type: LCMSMS

To describe an accurate and precise high-throughput analytical technique for the analysis of methylmalonic acid (MMA) utilizing Thermo Scientific SOLAµ WAX followed by LC-MS/MS. This technique further separates MMA from the known endogenous interference succinic acid (SA), a compound that has the same molecular weight as MMA and demonstrates a similar fragmentation pattern, which can cause issues with selectivity in mass spectrometry (MS) detection. For Research Use Only. Not for use in diagnostic procedures.

The application of SOLAμ for reduced sample volume analysis demonstrated with Niflumic acid.

Instrument Type: LCMS

The Thermo Scientific Dionex Ultimate 3000 system is applied to demonstrate how the Thermo Scientific SOLAμ SPE product can be used to facilitate the scale down of an extraction method when sample volume is limited. The Thermo Scientific Accucore HPLC column provided fast and efficient separation without the need for an ultra high pressure system. MS/MS detection was performed on a Thermo Scientific TSQ Vantage mass spectrometer. For Research Use Only. Not for use in diagnostic procedures.