The Thermo Scientific HPLC system is applied for the analysis of beta-blockers. The separation is performed using a Thermo Scientific Acclaim 120 C8 column, with UV detection at 214 nm.

Metoprolol succinate, a selective beta1-adrenoreceptor antagonist, reduces chest pain and lowers high blood pressure. Impurity profiling of a drug substance is important since even at small amounts they may influence product efficacy and safety. Metoprolol analysis by LC/UV detection has been described but some impurities do not possess a chromophore. The European Pharmacopeia specifies thin layer chromatography for impurities M and N analysis. This does not produce reliable quantitative data. The USP monograph modernization program indicates that a chromatographic method is more desirable.

The Thermo Scientific HPLC system is applied for the determination of six beta blockers. The separation is performed on a Thermo Scientific Accucore RP-MS HPLC column, with UV detection at 220 nm.

Thermo Scientific HyperSep solid-supported liquid-liquid extraction (SLE) is compared to traditional liquid-liquid extraction (LLE) for the extraction of beta blockers. The analysis is performed on a Thermo Scientific HPLC system using a Thermo Scientific Accucore C18 HPLC column. By using Thermo Scientific HyperSep SLE instead of traditional LLE methods, recovery of the analytes can be improved and the extraction time significantly reduced. For Research Use Only. Not for use in diagnostic procedures.

The Thermo Scientific HPLC system is applied for the analysis of beta blockers from urine. The samples are prepared using Thermo Scientific SOLA CX solid phase extraction (SPE) cartridges, and the separation is performed on a Thermo Scientific Accucore C18 HPLC column in less than 3 minutes, with UV detection at 220 nm. For Research Use Only. Not for use in diagnostic procedures.