The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Hydrophobic Interaction (HIC) analysis of Bevacizumab. The separation was performed on a Thermo Scientific MabPac HIC-20 column at UV detection of 214 nm. With a total run time of 40 minutes the method allows separation of all oxidized and deamidated impurities of Bevacizumab.

The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Hydrophobic Interaction (HIC) analysis of Rituximab. The separation was performed on a Thermo Scientific MabPac HIC-20 column at UV detection of 214 nm. With a total run time of 38 minutes the method allows separation of all oxidized and deamidated impurities of Rituximab.

The Thermo Scientific Dionex UltiMate 3000 Bio-LC system is applied for the reverse phase analysis of Rituximab (Drug Substance sample). The separation was performed on a Thermo Scientific MAbPac RP column. with UV detection at 214 nm. With a total run time of 70 minutes the method allows separation of all oxidized and deamidated impurities of Rituximab

The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Reverse phase analysis of Denosumab. The separation was performed on a Thermo Scientific MabPac RP column with a UV detection of 214 nm. With a run time of 80 minutes, the method allows easy separation of oxidized and Deamidated impurities.

The Thermo Scientific UltiMate 3000 Titanium HPLC system is applied to monitor the products of protein deamidation. The separation is performed on a Thermo Scientific ProPac WCX-10 column, with UV detection at 280 nm.