Tobramycin is an important aminoglycoside antibiotic used in ophthalmic and intravenous treatments to treat bacterial infections by blocking protein synthesis.[1] Determinations of tobramycin, kanamycin and other aminoglycoside antibiotics have been previously demonstrated by HPAE-IPAD since 2004.[2–6] This document updates Thermo Scientific™ Application Note 61 using a highpressure-capable Dionex Integrion HPIC system equipped with updated EG technology and an electrochemical detector.

Many drug substances are in salt forms to promote solubility, stability, and bioavailability. It is important to accurately determine the concentration of the counter ions to establish the correct molecular mass, the stoichiometric relationship and the completeness of salt formation. This application note describes the linearity, detection limits, precisions, and recoveries using anion-exchange chromatography with an electrolytically-generated potassium hydroxide eluent and IonPac AS18 column for the determination of sulfate counter ions and impurities in aminoglycosides.

Many drug substances are in salt forms to promote solubility, stability, and bioavailability. It is important to accurately determine the concentration of the counter ions to establish the correct molecular mass, the stoichiometric relationship and the completeness of salt formation. This application note describes the linearity, detection limits, precisions, and recoveries using anion-exchange chromatography with an electrolytically-generated eluent. Here is the impurities method using IonPac AS11-HC column for the determination of sulfate counter ions and impurities in aminoglycosides.

Paromomycin is an aminoglycoside antibiotic produced by Streptomyces rimosus var. paromomycinus. This Application Note demonstrates the use of an electrolytically generated potassium hydroxide eluent combined with the CarboPac PA1 anion-exchange column and IPAD using a disposable AAA Au working electrode for the determination of paromomycin in a bulk pharmaceutical formulation.

In this application note, we combine the CarboPac PA1, an eluent generator with CR-ATC, and disposable Au working electrodes to demonstrate an improved HPAE-PAD technology for Neomycin B purity analysis and its determination in Neosporin topical ointment, a complex over-the-counter pharmaceutical formulation.