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Displaying 1-7 of 7 results for: intact mass

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Intact mass analysis of monoclonal antibody charge variants by multi heart-cut 2D-LC/MS coupling ion-exchange and reversed-phase chromatography

Instrument Type: LCMS

Multiple heart-cut 2D-LC-MS is an efficient approach for charge variant analysis of antibodies by enabling direct MS analysis of first dimension ion-exchange fractions after desalting in the second dimension. Intact mass analysis facilitates identification and monitoring of mAb charge variant patterns. Annotations of major and minor charge variant peaks could be proposed. Enrichment of one fraction was used to improve the MS signal intensity.

AN73988: An Improved Method for Galactosyl Oligosaccharide Characterization

Instrument Type: ICMS

Here, we describe a HPAE-PAD/MS method for high-resolution profiling of GOS. Separation of individual oligosaccharides in GOS was achieved on a Thermo Scientific™ Dionex™ CarboPac™ PA300-4μm column. For mass spectrometry, the oligosaccharides pass through a desalting device that replaces sodium present in the eluent for hydronium ions. Intact oligosaccharides are first analyzed without fragmentation. Then, oligosaccharide fragmentation in the negative ionization mode provides MS2 spectra that include glycosidic as well as cross-ring fragments that that were used to identify oligosaccharide DP.

High-throughput LCMS analysis of oligonucleotides

Instrument Type: LCMS

High-throughput analysis of oligonucleotides using a single quadrupole mass spectrometer for quality control

A pre-concentration and on-line solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures

Instrument Type: LCMS

This study shows a fully automated UHPLC setup applying a simple and efficient online solid phase extraction method. Showcase assay performance by using a column cartridge for sample trapping and a Thermo Scientific™ MAbPac™ RP column for separation with water/acetonitrile-based gradients. Leverage the new technologies such as the Thermo Scientific™ Vanquish™ UHPLC platform in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole Orbitrap mass spectrometer.

Quantitative Forensic Analysis of Opiates, Opioids, and Their Metabolites in Human Urine Without Hydrolysis

Instrument Type: LCMSMS

Analysis of opiate and opioid metabolites in urine is most often done with a hydrolysis step that make total sample preparation time up to 24 hours. The method described here eliminates the hydrolysis step by analyzing the conjugated metabolites intact using a Thermo Scientific Prelude SPLC system for sample preparation and a Thermo Scientific TSQ Endura triple quadrupole mass spectrometer for analysis. For forensic use only.

Top Down Milk Protein Identification and Relative Quantification by Q Exactive Mass Spectrometer

Instrument Type: LCMSMS

Whey proteins, most notably alpha-lactalbumin and beta-lactoglobulin, are currently being investigated for their potential positive health benefits. Whey proteins are a substantial percentage of human milk (~60%) while such proteins are less abundant in cow milk (~20%). We describe a method for the identification of the various forms of caseins and whey proteins as a first step and then intact protein-based method for determination of the whey vs. casein protein ratio.

Fast Online Desalting of mAbs Using a Reversed-Phase Desalting Cartridge for LC-MS Analysis

Instrument Type: LCMS

This collateral describes a LCMS method for high throughput intact antibody characterization, capable of delivering the intact mass and the relative glycoform abundance of a mAb within 5 minutes.The desalting method optimization was conducted on a 2.1 × 10 mm Thermo Scientific™ MSPac™ DS-10 cartridge using conventional water/acetonitrile-based separations on the Thermo Scientific™ Vanquish™ UHPLC system and MS detection using the Thermo Scientific™ Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ mass spectrometer.