a1 A-1a A-1b a2 A-2 a3 Acidic Component 1 Acidic Component 2 Acidic Component 3 Acidic Component 4 Acidic Component 5 Acidic Variant-1 Acidic Variant-2 Acidic Variant-3 Acidic Variant-4 Acidic Variant-5 Acidic Variant-6 Acidic Variant-7 Acidic Variant-8 Acidic Variant-9 b1 B-1a B-1b B-1c B2 B-2 B-3 B-4 Basic Component 1 Basic Component 2 Basic Component 3 Basic Component 4 Basic Component 5 Basic Component 7 Basic Component 8 Basic Variant-1 Basic Variant-2 Basic Variant-3 Basic Variant-4 Basic Variant-5 Basic Variant-6 Basic Variant-7 Basic Variant-8 Bevacizumab cetuximab Component 1 Component 10 Component 11 Component 2 Component 3 Component 4 Component 5 Component 6 Component 7 Component 8 Component 9 Denosumab G0_G0F G0F_G0F G0F_G1F G1F_G1F G1F_G2F host cell material IgG-1 Infliximab M M-1a M-1b M-1c mAb NISTmAB (RM 8671) PostPeak 1 Pre Peak 1 Pre Peak 2 Pre Peak 3 Pre Peak 4 PrePeak 1 PrePeak 2 PrePeak 3 PrePeak 4 PrePeak 5 PrePeak 6 PrePeak 7 Ranibizumab Romiplostim Tocilizumab trastuzumab

1x deamidation 1x Lys 1x Lys, 1x deamidation 1x Lys, sialylated glycans 2x Lys 2x Lys, sialylated glycans acidic mAb variant basic mAb variant Charge Variant Charge Variant. IgG1 IgG-1 IgG1 Mab IgG-2 impurities Impurity mAb mAb variant Main Peak monoclonal antibody no modification Peptibody protein sialylated glycans