The Thermo Scientific HPLC system is applied for the analysis of vitamin K1 and K2. The separation is performed on a Thermo Scientific Acclaim HILIC-10 column, with UV detection at 265 nm.
The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of fat-soluble vitamins. The separation is performed using a Thermo Scientific Acclaim Phenyl-1 column, with UV detection at 220 nm.
The Thermo Scientific Ultimate 3000 system is applied for the improved separation of fat-soluble vitamins. The Thermo Scientific Acclaim RSLC PolarAdvantage column separated nine vitamins with a run time of less than 20 minutes.
In this work the Thermo Scientific Acclaim C30 column provides good resolution between both vitamin K1 and vitamin K2 and corresponding isomers, while the C18 column fails to separate these isomers.
The Thermo Scientific HPLC system is applied for the determination of vitamin K isomers. The separation is performed on a Thermo Scientific Accucore C30 HPLC column, with UV detection at 250 nm.
