A robust, sensitive, and reliable multi-residue LC-MS/MS method workflow solution that uses the Thermo Scientific TSQ Altis triple quadrupole mass spectrometer provides analysis and quantitation of more than 160 veterinary drugs with the Thermo Scientific VetDrugs Explorer Collection solution in salmon (fillet), bovine muscle, and milk. The complete instrument and data processing method including SRM settings is included with the VetDrugs Explorer Collection start-to-finish workflow solution. In 17 minutes, the target veterinary drugs were detected and quantified in food matrices.
The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the assay and organic impurities analysis of Tetracycline. The analysis was performed on a Thermo Scientific Acclaim Polar Advantage II C18 HPLC column using the method described in the USP 39 monograph. The results obtained for peak resolution exceed the criteria stated in the USP. Resolution between epitetracycline and tetracycline was 6.5 (USP criteria of NLT 2.5) and anhydrotetracycline and 4-epianhydrotetracycline was 8.7 (USP criteria of NLT 2.5). The tailing for tetracycline was 1.0 (USP criteria of NMT 1.5)
There is increasing demand to provide rapid and selective screening techniques for a wide range of antibiotic drugs. Their abuse as a feed supplement in food animals is of growing concern and is subject to regulations globally. Creating screening methods for multiple analytes is more cost effective than dedicated methods for fewer analytes. This application shows the advantages of using the Thermo Scientific Accucore Vanquish C18 UHPLC 1.5 µm column and Vanquish UHPLC system for the analysis of thirty-six antibiotics.
There is growing environmental concern regarding the health impact of trace levels of pharmaceuticals and personal care products (PPCPs) in water resources. In response, the U.S. Environmental Protection Agency (EPA) published Method 1694, which determines dozens of PPCPs in water, soil, sediment, and biosolids by HPLC-MS/MS. We demonstrate a progressive approach to analyzing PPCPs in environmental sources of water at the ng/L level with online sample preparation using small volumes of water, thus saving time and reducing the cost of analysis.
There is a growing concern over the presence of antibiotics in environmental sources of water. This has caused environmental and government labs to develop LC/MS methods to monitor water supplies for the presence of antibiotics. We developed a method to screen for antibiotics in surface water by applying on-line preconcentration and analyte extraction with LC/MS/MS detection.
