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Assay and organic impurities analysis of tetracycline using a polar embedded C18 HPLC column as per USP 39 monograph method Be the first to rate this application


The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the assay and organic impurities analysis of Tetracycline. The analysis was performed on a Thermo Scientific Acclaim Polar Advantage II C18 HPLC column using the method described in the USP 39 monograph. The results obtained for peak resolution exceed the criteria stated in the USP. Resolution between epitetracycline and tetracycline was 6.5 (USP criteria of NLT 2.5) and anhydrotetracycline and 4-epianhydrotetracycline was 8.7 (USP criteria of NLT 2.5). The tailing for tetracycline was 1.0 (USP criteria of NMT 1.5)
Market: Pharma
Keywords: HPLC, Tetracycline, UV detection, USP 39 monograph, Acclaim Polar Advantage-II C18
Matrix: N/A
Author: Krishnarao Dara
Affiliation: Thermo Fisher Scientific, CCS CoE Lab, Ahmedabad, India
Uploaded on 6/29/2017.

For Research Use Only. Not for use in diagnostic procedures.