Assay and organic impurities analysis of tetracycline using a polar embedded C18 HPLC column as per USP 39 monograph method
DescriptionThe Thermo Scientific Dionex UltiMate 3000 LC system is applied for the assay and organic impurities analysis of Tetracycline. The analysis was performed on a Thermo Scientific Acclaim Polar Advantage II C18 HPLC column using the method described in the USP 39 monograph. The results obtained for peak resolution exceed the criteria stated in the USP. Resolution between epitetracycline and tetracycline was 6.5 (USP criteria of NLT 2.5) and anhydrotetracycline and 4-epianhydrotetracycline was 8.7 (USP criteria of NLT 2.5). The tailing for tetracycline was 1.0 (USP criteria of NMT 1.5)
|Keywords:||HPLC, Tetracycline, UV detection, USP 39 monograph, Acclaim Polar Advantage-II C18|
|Affiliation:||Thermo Fisher Scientific, CCS CoE Lab, Ahmedabad, India|
For Research Use Only. Not for use in diagnostic procedures.