To demonstrate the application of a software-based method development approach for the advanced separation of all known water-soluble vitamins in reversed-phase mode with ultraviolet detection. To achieve transferability of the predicted method with excellent agreement of predicted and experimental retention times.
The Thermo Scientific Hypersil GOLD HILIC column was used to separate water soluble vitamins using UV detection at 205, 230 and 260 nm in under 8 minutes.
The Thermo Scientific HPLC system is applied for the analysis of vitamins and other ingredients in an energy drink. The separation is performed on a Thermo Scientific Acclaim PolarAdvantage (PA) column, with UV detection.
The Thermo Scientific Dionex UltiMate 3000 RSLC is applied for the analysis of water soluble vitamins from soft drink. The separation was performed in less than 5 minutes on a Thermo Scientific Acclaim RSLC PolarAdvantage II (PA2) 2.2 µm column with UV detection.
The Thermo Scientific Dionex UltiMate 3000 RS system is applied for the analysis of seven water-soluble vitamins. The separation was performed in less than 12 minutes on a Thermo Scientific Acclaim C30 5 µm column, with UV detection at 210 nm.
