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Accelerated method development for the separation of water-soluble vitamins by RP-HPLC with UV detection using an empirical approach to predict separation
Description
To demonstrate the application of a software-based method development approach for the advanced separation of all known water-soluble vitamins in reversed-phase mode with ultraviolet detection. To achieve transferability of the predicted method with excellent agreement of predicted and experimental retention times.| Market: | Food and Beverage |
| Keywords: | UV, Water-soluble vitamins, UHPLC, empirical approach, retention time prediction, reversed-phase chromatography, Vanquish Horizon, ultraviolet detection |
| Matrix: | Standard |
| Author: | Sylvia Grosse, Soo Hyun Park, Mauro De Pra, Frank Steiner |
| Affiliation: | Thermo Fisher Scientific, Germering, Germany |
