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Accelerated method development for the separation of water-soluble vitamins by RP-HPLC with UV detection using an empirical approach to predict separation
| # | Compound Name | Compound Class | RT (min) |
|---|---|---|---|
| 1 | Ascorbic acid | water-soluble vitamin | 0.99 |
| 2 | Nicotinic acid | water-soluble vitamin | 1.48 |
| 3 | Thiamine | water-soluble vitamin | 1.69 |
| 4 | Pyridoxal | water-soluble vitamin | 2.05 |
| 5 | Pyridoxine | water-soluble vitamin | 2.40 |
| 6 | Nicotinamide | water-soluble vitamin | 2.89 |
| 7 | Panthotenic acid | water-soluble vitamin | 3.11 |
| 8 | Folic acid | water-soluble vitamin | 5.79 |
| 9 | Biotin | water-soluble vitamin | 6.14 |
| 10 | Cyanocobalamin | water-soluble vitamin | 9.26 |
| 11 | Riboflavin | water-soluble vitamin | 10.70 |
Description
To demonstrate the application of a software-based method development approach for the advanced separation of all known water-soluble vitamins in reversed-phase mode with ultraviolet detection. To achieve transferability of the predicted method with excellent agreement of predicted and experimental retention times.| Market: | Food and Beverage |
| Keywords: | UV, Water-soluble vitamins, UHPLC, empirical approach, retention time prediction, reversed-phase chromatography, Vanquish Horizon, ultraviolet detection |
| Matrix: | Standard |
| Author: | Sylvia Grosse, Soo Hyun Park, Mauro De Pra, Frank Steiner |
| Affiliation: | Thermo Fisher Scientific, Germering, Germany |
