This study shows a fully automated UHPLC setup applying a simple and efficient online solid phase extraction method. Showcase assay performance by using a column cartridge for sample trapping and a Thermo Scientific™ MAbPac™ RP column for separation with water/acetonitrile-based gradients. Leverage the new technologies such as the Thermo Scientific™ Vanquish™ UHPLC platform in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole Orbitrap mass spectrometer.
Size exclusion chromatography (SEC) is one of the major analyses in biotherapeutics characterization. Here we show a MAbPac SEC-1 column test with the Thermo Scientific Vanquish Flex UHPLC system.
The Thermo Scientific HPLC system is applied for the analysis of protein standards. The separation is performed on a Thermo Scientific Acclaim 300 C18 micro LC column, with UV detection at 214 nm.
The Thermo Scientific HPLC system is applied for the analysis of a protein mixture. The separation is performed on a Thermo Scientific Acclaim 300 C18 column, with UV detection at 214 nm.
The Thermo Scientific HPLC system is applied for the analysis of intact proteins (*indicates an impurity from carbonic anhydrase). The separation is performed on a Thermo Scientific Accucore 150-C4 150 Å pore diameter nanoLC column, with UV detection at 214 nm. The 150 Å pore size enables the effective analysis of molecules unable to penetrate into smaller diameter pores, whilst the low hydrophobicity C4 phase results in protein separation by hydrophobicity.
