Choline is a water soluble essential nutrient that is produced in the body in small quantities. Choline is a precursor of acetylcholine which is an important neurotransmitter involved in muscle control and memory. Choline is a small, highly polar quaternary amine and will not be retained using a C18 column. The zwitterionic nature of Syncronis HILIC makes it ideal for the retention of this class of compound. This application note demonstrates the successful analysis of acetylcholine and choline using a Syncronis HILIC 1.7 µm column.
Choline and acetylcholine are quaternary ammonium cations which are noted for their activities as neurotransmitters. This is an older application note that shows these compounds can be determined by IC. Today we would probably use an IonPac CS19 column as shown in Application Update 189.
This work presents IC-based methods for the detection and quantification of methacholine and possible impurities in solutions used to assess bronchial asthma of subjects evaluated in respiratory function labs and epidemiological field studies. Methacholine assay involves diluting the original solution (dissolved in 0.9% sodium chloride with or without 0.4% phenol) 1000-fold with deionized water, then analyzing the sample on a cation RFIC system. These methods demonstrate high precision and accuracy for both methacholine and possible impurities present in amounts approaching 1%.
The Thermo Scientific Dionex Ultimate 3000 RSLC system with quaternary LPG is applied for the analysis of anesthetic and muscle relaxants. The separation was performed in less than 12 minutes on a Thermo Scientific Dionex Acclaim Trinity P1 3 µm column with a Thermo Scientific Dionex Corona Ultra charged aerosol detector (CAD).
The Thermo Scientific HPLC system is applied for the analysis of choline and acetylcholine. The separation is performed on a Thermo Scientific Syncronis HILIC 1.7 μm HPLC column in less than 2.5 minutes, with MS/MS detection.
