This application demonstrates the straightforward transfer of an analytical HPLC method from an Agilent 1260 Infinity instrument to a Thermo Scientific UltiMate 3000 HPLC system and a Thermo Scientific Vanquish Flex UHPLC system with the example of a USP derived assay for the analysis of acetaminophen and its impurities.
This application demonstrates the straightforward transfer of an analytical HPLC method from a Thermo Scientific UltiMate 3000 HPLC system to a Thermo Scientific Vanquish Flex UHPLC system demonstrated with a USP derived assay for the analysis of acetaminophen and its impurities.
This application demonstrates the utilization of the Thermo Scientific Vanquish Flex Duo UHPLC system for Dual LC for the parallel implementation of independent HPLC and UHPLC methods at two independent flow paths in one instrument stack. For illustration the speed-up of a legacy HPLC method for acetaminophen impurity analysis to an optimized UHPLC method is described.
The Thermo Scientific Dionex Ultimate 3000 system is applied for the accurate assay and impurity analysis of paracetamol tablets. The separation is performed on a Thermo Scientific Syncronis C18 HPLC column in less than 20 minutes, with UV detection at 245 and 275 nm.
The Thermo Scientific Dionex UltiMate 3000 HPLC system is applied for the analysis of paracetamol, caffeine and their impurities. The Thermo Scientific Syncronis C18 5 µm column separated the 5 compounds in less than 13 minutes with resolution between paracetamol and theophylline greater than 3.
