TN72225: Glycoprotein Monosaccharide Analysis using HPAE-PAD with Manually Prepared Eluent
DescriptionThis work describes an HPAE-PAD method for monosaccharide composition analysis using manually prepared eluent. Monosaccharide analysis using electrolytically generated eluent has been described before. Here, three commercially available proteins, IgG, fetuin, and alpha-1-acid glycoprotein, were individually subjected to two hydrolysis conditions using 1)HCl, for the amino sugars galactosamine and glucosamine, and 2)TFA, for the neutral sugars mannose, glucose, and galactose. Results for method linearity, robustness, and accuracy are presented here. An ICS-6000 can be used for this application.
|Keywords:||Electrochemical detection, Glycoproteins, ICS-5000+, IgG, mAb, HPAE-PAD, CarboPac PA20 Column, Dionex AminoTrap column, Manual eluent preparation, Biopharmaceuticals, Quality control, ICS-6000|
|Matrix:||Glycoprotein acid digest|
|Author:||Sachin Patil and Jeffrey Rohrer|
|Affiliation:||Thermo Fisher Scientific, Sunnyvale, CA, USA|
For Research Use Only. Not for use in diagnostic procedures.