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TN72225: Glycoprotein Monosaccharide Analysis using HPAE-PAD with Manually Prepared Eluent Be the first to rate this application


This work describes an HPAE-PAD method for monosaccharide composition analysis using manually prepared eluent. Monosaccharide analysis using electrolytically generated eluent has been described before. Here, three commercially available proteins, IgG, fetuin, and alpha-1-acid glycoprotein, were individually subjected to two hydrolysis conditions using 1)HCl, for the amino sugars galactosamine and glucosamine, and 2)TFA, for the neutral sugars mannose, glucose, and galactose. Results for method linearity, robustness, and accuracy are presented here. An ICS-6000 can be used for this application.
Market: BioPharma
Keywords: Electrochemical detection, Glycoproteins, ICS-5000+, IgG, mAb, HPAE-PAD, CarboPac PA20 Column, Dionex AminoTrap column, Manual eluent preparation, Biopharmaceuticals, Quality control, ICS-6000
Matrix: Glycoprotein acid digest
Author: Sachin Patil and Jeffrey Rohrer
Affiliation: Thermo Fisher Scientific, Sunnyvale, CA, USA
Uploaded on 3/27/2017.

For Research Use Only. Not for use in diagnostic procedures.