A high resolution separation of 2-AA derivatized N-Glycans from a commercial chimeric IgG1 monoclonal antibody (Infliximab)
DescriptionThe Thermo Scientific Vanquish H UHPLC system was used for the high resolution determination of 2-AA (anthranilic acid) labelled N-glycans released from a commercial chimeric IgG1 mAb (Infliximab). The separation is performed on a 150 mm Accucore 150-Amide-HILIC column with fluorescence detection giving separation in less than 30 minutes.N-Glycans present at the asparagine 297 residue in the CH2 domain of the Fc region of IgG play a crucial role in modulating the structural stability and functional activity relationship of the antibody.
|Keywords:||Biotherapeutics, Glycosylation, Fluorescence detection, N-glycans, Monoclonal Antibodies, Infliximab, 2-AA derivatives, NIBRT, Bioprocessing Research|
|Author:||Silvia Millán, Stefan Mittermayr, Amy Farrell and Jonathan Bones|
|Affiliation:||NIBRT, Dublin, Ireland|
|Status:||Proof of Concept|
For Research Use Only. Not for use in diagnostic procedures.