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A high resolution separation of 2-AA derivatized N-Glycans from a commercial chimeric IgG1 monoclonal antibody (Infliximab)
Description
The Thermo Scientific Vanquish H UHPLC system was used for the high resolution determination of 2-AA (anthranilic acid) labelled N-glycans released from a commercial chimeric IgG1 mAb (Infliximab). The separation is performed on a 150 mm Accucore 150-Amide-HILIC column with fluorescence detection giving separation in less than 30 minutes.N-Glycans present at the asparagine 297 residue in the CH2 domain of the Fc region of IgG play a crucial role in modulating the structural stability and functional activity relationship of the antibody.| Market: | BioPharma |
| Keywords: | Biotherapeutics, Glycosylation, Fluorescence detection, N-glycans, Monoclonal Antibodies, Infliximab, 2-AA derivatives, NIBRT, Bioprocessing Research |
| Matrix: | Drug product |
| Author: | Silvia Millán, Stefan Mittermayr, Amy Farrell and Jonathan Bones |
| Affiliation: | NIBRT, Dublin, Ireland |
| Status: | Proof of Concept |
For Research Use Only. Not for use in diagnostic procedures.
