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A high resolution separation of 2-AA derivatized N-Glycans from a commercial chimeric IgG1 monoclonal antibody (Infliximab) Be the first to rate this application


The Thermo Scientific Vanquish H UHPLC system was used for the high resolution determination of 2-AA (anthranilic acid) labelled N-glycans released from a commercial chimeric IgG1 mAb (Infliximab). The separation is performed on a 150 mm Accucore 150-Amide-HILIC column with fluorescence detection giving separation in less than 30 minutes.N-Glycans present at the asparagine 297 residue in the CH2 domain of the Fc region of IgG play a crucial role in modulating the structural stability and functional activity relationship of the antibody.
Market: BioPharma
Keywords: Biotherapeutics, Glycosylation, Fluorescence detection, N-glycans, Monoclonal Antibodies, Infliximab, 2-AA derivatives, NIBRT, Bioprocessing Research
Matrix: Drug product
Author: Silvia Millán, Stefan Mittermayr, Amy Farrell and Jonathan Bones
Affiliation: NIBRT, Dublin, Ireland
Status: Proof of Concept
Uploaded on 12/14/2016.

For Research Use Only. Not for use in diagnostic procedures.