A fast separation of 2-AB labelled N-Glycans from polyclonal human serum IgG
DescriptionThe Thermo Scientific Vanquish H UHPLC system is applied for the rapid determination of 2-AB (2-aminobenzamide) labelled N-glycans released from human serum IgG in less than 15 minutes using a 50 mm Thermo Scientific Accucore 150-Amide-HILIC column with fluorescence detection. The method is able to determine N-Glycans present at the asparagine 297 residue in the CH2 domain of the Fc region of IgG that play a crucial role in modulating the structural stability and functional activity relationship of the antibody.
|Keywords:||Biotherapeutics, Glycosylation, Human IgG, Fluorescence detection, N-glycans, Monoclonal Antibodies, 2-AB derivatives, NIBRT, Bioprocessing Research|
|Matrix:||Human IgG serum|
|Author:||Silvia Millán, Stefan Mittermayr, Amy Farrell and Jonathan Bones|
|Affiliation:||NIBRT, Dublin, Ireland|
For Research Use Only. Not for use in diagnostic procedures.