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A fast separation of 2-AB labelled N-Glycans from polyclonal human serum IgG
(1)

Description

The Thermo Scientific Vanquish H UHPLC system is applied for the rapid determination of 2-AB (2-aminobenzamide) labelled N-glycans released from human serum IgG in less than 15 minutes using a 50 mm Thermo Scientific Accucore 150-Amide-HILIC column with fluorescence detection. The method is able to determine N-Glycans present at the asparagine 297 residue in the CH2 domain of the Fc region of IgG that play a crucial role in modulating the structural stability and functional activity relationship of the antibody.
Market: BioPharma
Keywords: Biotherapeutics, Glycosylation, Human IgG, Fluorescence detection, N-glycans, Monoclonal Antibodies, 2-AB derivatives, NIBRT, Bioprocessing Research
Matrix: Human IgG serum
Author: Silvia Millán, Stefan Mittermayr, Amy Farrell and Jonathan Bones
Affiliation: NIBRT, Dublin, Ireland
Uploaded on 12/13/2016.

For Research Use Only. Not for use in diagnostic procedures.