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A fast separation of 2-AB labelled N-Glycans from polyclonal human serum IgG
Description
The Thermo Scientific Vanquish H UHPLC system is applied for the rapid determination of 2-AB (2-aminobenzamide) labelled N-glycans released from human serum IgG in less than 15 minutes using a 50 mm Thermo Scientific Accucore 150-Amide-HILIC column with fluorescence detection. The method is able to determine N-Glycans present at the asparagine 297 residue in the CH2 domain of the Fc region of IgG that play a crucial role in modulating the structural stability and functional activity relationship of the antibody.| Market: | BioPharma |
| Keywords: | Biotherapeutics, Glycosylation, Human IgG, Fluorescence detection, N-glycans, Monoclonal Antibodies, 2-AB derivatives, NIBRT, Bioprocessing Research |
| Matrix: | Human IgG serum |
| Author: | Silvia Millán, Stefan Mittermayr, Amy Farrell and Jonathan Bones |
| Affiliation: | NIBRT, Dublin, Ireland |
For Research Use Only. Not for use in diagnostic procedures.
