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Setting the correct UHPLC instrument connections for protein aggregate analysis by size-exclusion chromatography
Description
The importance of correct UHPLC instrument setup for protein aggregate analysis by size-exclusion chromatography is shown by using the Thermo Scientific Vanquish Flex Quaternary UHPLC system. For this analysis, pre-column dispersion was reduced to show the effects of dispersion in front of the column at different flow rates. After the flow rates are reduced to 300 μL/min with the MAbPac-SEC-1 column formats of 4 mm i.d., the use of low volume tubing and fittings becomes essential in order to maintain resolution of aggregates from the main peak. Refer to attached pdf file for full experiment.| Market: | BioPharma |
| Keywords: | Monoclonal antibody, Size-Exclusion Chromatography, mAb, pre-column dispersion, protein aggregation analysis, biotherapeutics,, NIBRT, Bioprocessing Research |
| Matrix: | N/A |
| Author: | Amy Farrell, Jonathan Bones, and Ken Cook |
| Affiliation: | NIBRT, Dublin, Ireland |
For Research Use Only. Not for use in diagnostic procedures.
