A universal chromatography method for aggregate analysis of monocolonal antibodies

Instrument Type: UHPLC

Analysis of protein aggregation of five important biotherapeutic monoclonal antibodies (mAbs) by size-exclusion chromatography, showing the universal applicability of the Thermo Scientific MAbPac SEC-1 column for aggregate analysis of mAbs. The biopharmaceutical industry continues to develop mAb-based biotherapeutics have several key quality attributes that require quantification. This shows the ability to determine quickly and accurately the percentage of aggregates and fragments for five therapeutics as shown with full details in the AN21601 download.

Setting the correct UHPLC instrument connections for protein aggregate analysis by size-exclusion chromatography

Instrument Type: UHPLC

The importance of correct UHPLC instrument setup for protein aggregate analysis by size-exclusion chromatography is shown by using the Thermo Scientific Vanquish Flex Quaternary UHPLC system. For this analysis, pre-column dispersion was reduced to show the effects of dispersion in front of the column at different flow rates. After the flow rates are reduced to 300 μL/min with the MAbPac-SEC-1 column formats of 4 mm i.d., the use of low volume tubing and fittings becomes essential in order to maintain resolution of aggregates from the main peak. Refer to attached pdf file for full experiment.

AN1058: Determination of Polyacrylic Acid in Nuclear Power Plant Pressurized Water Reactor Secondary Feed Water

Instrument Type: IC

Polyacrylic acid (PAA) has been used as a dispersant in the feed water to reduce the accumulation of metal oxide deposits on the secondary-side surfaces of the steam generators at nuclear power plants. This application describes a size-exclusion chromatography with UV detection method to determine low concentrations of PAA in secondary feed water containing ethanolamine (ETA) and hydrazine.