Find methods for your needs

Determination of Lactose and Protein by 2D-HPLC Be the first to rate this application

Description

Many biopharmaceutical formulations use carbohydrates to enhance the stability of proteins. This method is an example of a "heart-cut" 2D HPLC method, separating the carbohydrate analyte from the protein. The method uses two charge aerosol detectors, as an example, but one can be substituted by UV. Calibration for the Sucrose is from 15.6 - 2000 ng o.c. If a second Corona Veo SD is used for the Protein, it uses the parameters described as "Veo SD".
Market: BioPharma
Keywords: carbohydrate, BSA, Bovine serum albumin, Lactose
Matrix: Water
Author: Marc Plante
Affiliation: Thermo Fisher Scientific
Uploaded on 12/1/2015.

For Research Use Only. Not for use in diagnostic procedures.