The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of Lactulose. The separation was performed on a Thermo Scientific Hypersil Gold amino HPLC column using the method as described in the USP 38 monograph. Resolution between Epilactose and Lactose Peak was 1.28 (met USP criteria of NLT 0.9), RRT for Epilactose with respect to Lactulose peak was 0.92 (met USP criteria of about 0.83), Resolution between Lactulose and Lactose Peak was 3.17 (met USP criteria of NLT 1.5), RRT for Lactose with respect to Lactulose peak was 1.22 (met USP criteria of about 1.1),

Many biopharmaceutical formulations use carbohydrates to enhance the stability of proteins. This method is an example of a "heart-cut" 2D HPLC method, separating the carbohydrate analyte from the protein. The method uses two charge aerosol detectors, as an example, but one can be substituted by UV. Calibration for the Sucrose is from 15.6 - 2000 ng o.c. If a second Corona Veo SD is used for the Protein, it uses the parameters described as "Veo SD".

In many of the vaccines and other formulations that are used in the prevention and treatment of many conditions and illnesses contain carbohydrates, as a part of their formulation. Six carbohydrates were identified as important components in biotechnological formulations: sorbitol, mannitol, sucrose, lactose, maltose, and trehalose. These are separated by HILIC-HPLC and detected by the universal Corona charged aerosol detector.

This Application Note describes the use of two different anion-exchange columns with amperometric detection to analyze simple sugars, sugar alcohols, alcohols, and glycols in yeast and bacterial fermentation broths. The yeast Saccharomyces cerevisiae in Yeast Extract-Peptone-Dextrose (YPD) broth and the bacteria Escherichia coli (E. coli) in Luria-Bertani (LB) broth are common eukaryotic and prokaryotic fermentation systems, respectively. This record shows a method that is best for mono- and disaccharides.

The separation of five sugars was performed using the Thermo Scientific Hypersil GOLD Amino column in HILIC mode with RI detection.