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Determination of Sucrose and Protein by 2D-HPLC
Description
Many biopharmaceutical formulations use carbohydrates to enhance the stability of proteins. This method is an example of a "heart-cut" 2D HPLC method, separating the carbohydrate analyte from the protein. The method uses two charge aerosol detectors, as an example, but one can be substituted by UV. Calibration for the Sucrose is from 15.6 - 2000 ng o.c. The detector labelled "Veo RS" can be a Veo SD run with evaporation temperature of 50C. If a second Corona Veo SD is used for the Protein, it uses the parameters described as "Veo SD".| Market: | BioPharma |
| Keywords: | Bovine Serum Albumin (BSA), carbohydrate, Protein, Sucrose |
| Matrix: | Water |
| Author: | Marc Plante |
| Affiliation: | Thermo Scientific |
For Research Use Only. Not for use in diagnostic procedures.
