Thermo Scientific AppsLab Library: Determination of Sucrose and Protein by 2D-HPLC

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Determination of Sucrose and Protein by 2D-HPLC Be the first to rate this application

#	Compound Name	Compound Class	RT (min)
1	BSA	Protein	9.80

Description

Many biopharmaceutical formulations use carbohydrates to enhance the stability of proteins. This method is an example of a "heart-cut" 2D HPLC method, separating the carbohydrate analyte from the protein. The method uses two charge aerosol detectors, as an example, but one can be substituted by UV. Calibration for the Sucrose is from 15.6 - 2000 ng o.c. The detector labelled "Veo RS" can be a Veo SD run with evaporation temperature of 50C. If a second Corona Veo SD is used for the Protein, it uses the parameters described as "Veo SD".

Market:	BioPharma
Keywords:	Bovine Serum Albumin (BSA), carbohydrate, Protein, Sucrose
Matrix:	Water
Author:	Marc Plante
Affiliation:	Thermo Scientific

Uploaded on 11/19/2015.

For Research Use Only. Not for use in diagnostic procedures.

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Determination of Sucrose and Protein by 2D-HPLC Be the first to rate this application

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