Determination of Sucrose and Protein by 2D-HPLC

Instrument Type: HPLC-CAD

Many biopharmaceutical formulations use carbohydrates to enhance the stability of proteins. This method is an example of a "heart-cut" 2D HPLC method, separating the carbohydrate analyte from the protein. The method uses two charge aerosol detectors, as an example, but one can be substituted by UV. Calibration for the Sucrose is from 15.6 - 2000 ng o.c. The detector labelled "Veo RS" can be a Veo SD run with evaporation temperature of 50C. If a second Corona Veo SD is used for the Protein, it uses the parameters described as "Veo SD".

Determination of Sorbitol and Protein by 2D-HPLC

Instrument Type: HPLC-CAD

In bioformulations containing a protein and a carbohydrates, carbohydrates are used to assist in the stabilization of the proteins in solution, and are used in different amounts, depending on the protein and the product requirements. Sorbitol, a sugar alcohol is transferred to and analysed by HILIC and CAD after it is eluted from the reverse-phase column. Both sorbitol and the protein are analysed in this 2D-HPLC method.