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TN36: Analysis of Exoglycosidase Digestions of N-Linked Oligosaccharides Using HPAE-PAD (Part B) Be the first to rate this application

analysis exoglycosidase digestions

Description

This technical note describes the use of exoglycosidases to obtain structural information about N-linked oligosaccharides commonly found in mammalian glycoproteins. HPAE-PAD is used to confirm the identity of the released monosaccharides, as well as the remaining di-, tri,- or oligosaccharides. Empirical rules used to predict oligosaccharide elution order are included in the last section of this technical note.
Market: BioPharma
Keywords: glycoprotein, HPAE-PAD, Endoglycosidase, N-linked oligosaccharide, TN71, TN 20, TN 70671, TN20, TN70671, TN 36
Matrix: Water
Affiliation: Thermo Fisher Scientific, Sunnyvale, CA USA
Uploaded on 10/29/2015.

For Research Use Only. Not for use in diagnostic procedures.