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AU176: Preparation of Peptide N-Glycosidase F Digests for HPAE-PAD Analysis Be the first to rate this application

pngase f digestion glycoproteins

Description

The work shown here describes glycoprotein deglycosylation with PNGase F, followed by an efficient sample pretreatment using desalting and detergent removal spin columns to remove high concentrations of salt and detergents from small-scale PNGase F digestions. The HPAE-PAD technique was used to separate and detect the oligosaccharides. Oligosaccharides were separated using the Dionex CarboPac PA200 column. The PA200 is a nonporous, high-efficiency, pellicular anion-exchange column that provides high-resolution separations required for oligosaccharide mapping and analysis.
Market: BioPharma
Keywords: CarboPac PA200, PNGase F, Protein Glycosylation, AU 176, N-glycans, O-glycans, HPAE-PAD, TN 20, TN 70671, TN20, TN70671
Matrix: Water
Author: Pranathi Perati and Jeff Rohrer
Affiliation: Thermo Fisher Scientific, Sunnyvale, CA USA
Uploaded on 8/31/2015.

For Research Use Only. Not for use in diagnostic procedures.