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AU176: Preparation of Peptide N-Glycosidase F Digests for HPAE-PAD Analysis
Description
The work shown here describes glycoprotein deglycosylation with PNGase F, followed by an efficient sample pretreatment using desalting and detergent removal spin columns to remove high concentrations of salt and detergents from small-scale PNGase F digestions. The HPAE-PAD technique was used to separate and detect the oligosaccharides. Oligosaccharides were separated using the Dionex CarboPac PA200 column. The PA200 is a nonporous, high-efficiency, pellicular anion-exchange column that provides high-resolution separations required for oligosaccharide mapping and analysis.| Market: | BioPharma |
| Keywords: | CarboPac PA200, PNGase F, Protein Glycosylation, AU 176, N-glycans, O-glycans, HPAE-PAD, TN 20, TN 70671, TN20, TN70671 |
| Matrix: | Water |
| Author: | Pranathi Perati and Jeff Rohrer |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
