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Displaying 1-3 of 3 results for Tag: Protein Glycosylation
AU176: Preparation of Peptide N-Glycosidase F Digests for HPAE-PAD Analysis
Instrument Type: ICThe work shown here describes glycoprotein deglycosylation with PNGase F, followed by an efficient sample pretreatment using desalting and detergent removal spin columns to remove high concentrations of salt and detergents from small-scale PNGase F digestions. The HPAE-PAD technique was used to separate and detect the oligosaccharides. Oligosaccharides were separated using the Dionex CarboPac PA200 column. The PA200 is a nonporous, high-efficiency, pellicular anion-exchange column that provides high-resolution separations required for oligosaccharide mapping and analysis.
AN1130: HPAE-FLD Method for Separation of 2-Aminobenzamide (2-AB)-Labeled Oligosaccharides from Human α1 Acid-Glycoprotein and Bovine Fetuin (Part B- Modified Elution)
Instrument Type: ICHere, oligosaccharides released from two glycoproteins are labeled with a fluorophore followed by their purification and HPAE-FLD analysis. Two different methods for purification of the labeled oligosaccharides from unbound labeling reagent were tested along with two elution conditions. The data show that the variations tested here do not significantly affect the retention time profiles and that the method described here is suitable for routine profiling of oligosaccharides released from glycoproteins. An ICS-6000 can be used for this application.
AN1130: HPAE‐FLD Method for Separation of 2‐Aminobenzamide (2‐AB)‐Labeled Oligosaccharides from Human α1 Acid‐Glycoprotein and Bovine Fetuin (Part A-Unmodified Elution).
Instrument Type: ICHere, oligosaccharides released from two glycoproteins are labeled with a fluorophore followed by their purification and HPAE-FLD analysis. Two different methods for purification of the labeled oligosaccharides from unbound labeling reagent were tested along with two elution conditions. The data show that the variations tested here do not significantly affect the retention time profiles and that the method described here is suitable for routine profiling of oligosaccharides released from glycoproteins. An ICS-6000 can be used for this application.
