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AN1130: HPAE‐FLD Method for Separation of 2‐Aminobenzamide (2‐AB)‐Labeled Oligosaccharides from Human α1 Acid‐Glycoprotein and Bovine Fetuin (Part A-Unmodified Elution).
Description
Here, oligosaccharides released from two glycoproteins are labeled with a fluorophore followed by their purification and HPAE-FLD analysis. Two different methods for purification of the labeled oligosaccharides from unbound labeling reagent were tested along with two elution conditions. The data show that the variations tested here do not significantly affect the retention time profiles and that the method described here is suitable for routine profiling of oligosaccharides released from glycoproteins. An ICS-6000 can be used for this application.| Market: | BioPharma |
| Keywords: | 2-AB labeling, Carbohydates, CarboPac PA1, ICS-5000+, AN 1130, Protein Glycosylation, PTM, N-linked Glycans, O-linked Glycans, ICS-6000, AU 206 |
| Matrix: | Water |
| Author: | Sachin Patil, John Guajardo, and Jeff Rohrer |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA |
For Research Use Only. Not for use in diagnostic procedures.
