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HIC-Analysis of a Mixture of Proteins and a mAb Be the first to rate this application

Description

Hydrophobic interaction liquid chromatography (HIC) is one of the major analyses in biotherapeutics characterization. Here we show a high resolution separation of proteins and a mAb in 20 minutes with the Thermo Scientific Vanquish Flex UHPLC system. The unique chemistry of the MAbPac HIC-20 column allows the analysis of hydrophilic proteins such as myoblogin and ribonuclease A as well as more hydrophobic mAbs.
Market: BioPharma
Keywords: protein characterization, MAbPac, Monoclonal antibody, Hydrophobic interaction liquid chromatography
Matrix: 100 mM sodium phosphate, 2 M ammonium sulfate, pH 7
Author: Michael Heidorn
Uploaded on 6/19/2015.

For Research Use Only. Not for use in diagnostic procedures.