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Displaying 1-5 of 5 results for Tag: protein characterization
Accurately Monitor Protein PEGylation Reactions by HPLC-UV-CADInstrument Type: HPLC-CAD
This application provides a fast and accurate way for biopharmaceutical chemists to monitor PEGylation reactions by using HPLC with complementary UV/Vis and charged aerosol detection.Separation on the MAbPac RP resolves PEGylation reagents, unreacted IgG, and PEGylated IgG products that elute as a broad hump.
MAbPac SEC-1 Column Test with Vanquish FlexInstrument Type: Unspecified
Size exclusion chromatography (SEC) is one of the major analyses in biotherapeutics characterization. Here we show a MAbPac SEC-1 column test with the Thermo Scientific Vanquish Flex UHPLC system.
HIC-Analysis of a Mixture of Proteins and a mAbInstrument Type: UHPLC
Hydrophobic interaction liquid chromatography (HIC) is one of the major analyses in biotherapeutics characterization. Here we show a high resolution separation of proteins and a mAb in 20 minutes with the Thermo Scientific Vanquish Flex UHPLC system. The unique chemistry of the MAbPac HIC-20 column allows the analysis of hydrophilic proteins such as myoblogin and ribonuclease A as well as more hydrophobic mAbs.
High Resolution Peptide Mapping for Biopharmaceutical AnalysisInstrument Type: HPLC (Biocompatible)
A sample of a monoclonal antibody, was separated by a Thermo Scientific UltiMate 3000 BioRS Dual System. The Thermo Scientific Acclaim RSLC 120 C18, 2.2 µm, 2.1x250 mm column was operated at 80 °C, to obtain high resolution separation of the complex mixture. Due to the high temperature in the column, the pre-heating of the mobile phase was essential to avoid thermal mismatch between the mobile and the column. A pre-column heater 2 µL biocompatiblewas used to achieve thermal equilibration between column and mobile phase.
Glycans by UHPLC-CAD on Thermo Scientific Dionex UltiMate 3000 RSLCInstrument Type: HPLC-CAD
Glycans released from bovine fetuin by PNGase-F are separated by gradient elution on the Thermo Scientific Dionex GlycanPac AXR-1 column and detected by charged aerosol detection on the Corona Veo RS.