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AU151: Determination of Sucralose in Reduced- Carbohydrate Colas using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection
Description
Sucralose (trichlorogalactosucrose or 1,6-dichloro- 1,6-dideoxy-b-D-fructofuranosyl-4-deoxy-a-D-galacto- pyranoside) is a non-nutritive sweetener used in beverages. Detection of sucralose is challenging because carbohydrates lack a strong chromophore. In contrast, high performance anion exchange (HPAE) chromatography with pulsed amperometric detection (PAD) is a sensitive, selective and direct method to determine carbohydrates. Sucralose in a reduced-carbohydrate carbonated beverage is retained longer and therefore well-resolved from fructose and sucrose on the CarboPac PA20 column.Market: | Food and Beverage |
Keywords: | CarboPac PA20, Glucose, Sucralose, Carbonated beverage, Sucrose, fructose, HPAE-PAD, TN 20, TN 70671, TN20, TN70671, AU 151 |
Matrix: | Carbonated beverage |
Author: | Terri Christison and Jeffrey Rohrer |
Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.