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AN148: Determination of Bethanechol by Ion Chromatography. Be the first to rate this application

determination bethanechol


Bethanechol chloride is a quaternary ammonium compound that is structurally and pharmacologically related to acetylcholine. A method in the USP 24 NF 19 recently proposed that the gravimetric assay for bethanechol chloride be replaced with a more specific and rugged ion chromatography assay. The proposed method specifies the use of a Dionex IonPac CS14 separator column using methanesulfonic acid (MSA) eluent and suppressed conductivity detection. Here, we applied electrolytic on-line generation of MSA to optimize reproducibility, convenience, and method transfer between laboratories.
Market: Pharma
Keywords: 2-HPTA, Bethanechol chloride, EG40 Eluent Generator, IonPac CS14, Monograph, Quaternary ammonium compound, RFIC, RFIC-EG system, Suppressed Conductivity Detection, USP
Matrix: Water
Author: David H. Thomas and Jeffrey S. Rohrer
Affiliation: Thermo Fisher Scientific, Sunnyvale, CA USA
Uploaded on 2/5/2015.

For Research Use Only. Not for use in diagnostic procedures.