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Displaying 1-2 of 2 results for Tag: Bethanechol chloride

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AN249: Determination of Methacholine Chloride and Potential Impurities Using a Reagent-Free Ion Chromatography System

Instrument Type: IC

This work presents IC-based methods for the detection and quantification of methacholine and possible impurities in solutions used to assess bronchial asthma of subjects evaluated in respiratory function labs and epidemiological field studies. Methacholine assay involves diluting the original solution (dissolved in 0.9% sodium chloride with or without 0.4% phenol) 1000-fold with deionized water, then analyzing the sample on a cation RFIC system. These methods demonstrate high precision and accuracy for both methacholine and possible impurities present in amounts approaching 1%.

AN148: Determination of Bethanechol by Ion Chromatography.

Instrument Type: IC

Bethanechol chloride is a quaternary ammonium compound that is structurally and pharmacologically related to acetylcholine. A method in the USP 24 NF 19 recently proposed that the gravimetric assay for bethanechol chloride be replaced with a more specific and rugged ion chromatography assay. The proposed method specifies the use of a Dionex IonPac CS14 separator column using methanesulfonic acid (MSA) eluent and suppressed conductivity detection. Here, we applied electrolytic on-line generation of MSA to optimize reproducibility, convenience, and method transfer between laboratories.