AU72197 : Determination of Tobramycin Using HPAE-IPAD on a Compact IC System: 20 µM tobramycin

Instrument Type: IC

Tobramycin is an important aminoglycoside antibiotic used in ophthalmic and intravenous treatments to treat bacterial infections by blocking protein synthesis.[1] Determinations of tobramycin, kanamycin and other aminoglycoside antibiotics have been previously demonstrated by HPAE-IPAD since 2004.[2–6] This document updates Thermo Scientific™ Application Note 61 using a highpressure-capable Dionex Integrion HPIC system equipped with updated EG technology and an electrochemical detector.

AU167: Determination of Tobramycin in Crude and In-Process Production Samples During Manufacturing Using HPAE-IPAD

Instrument Type: IC

This update describes how tobramycin and its typical impurities can be determined in manufacturing process matrices using the same HPAE-IPAD method described in AN 61. The same method is used to assay tobramycin that is intentionally degraded, demonstrating its suitability as a stability indicating method. An ICS-6000 can be used for this application.

AN61: Determination of Tobramycin and Impurities Using HPAE-PAD

Instrument Type: IC

In this application note, we combine the CarboPac PA1, eluent generator with CR-ATC, and disposable Au working electrodes to demonstrate an improved HPAE-PAD technology for tobramycin purity analysis.

AN267: Analysis of the Aminoglycoside Antibiotics Kanamycin and Amikacin Matches USP Requirements

Instrument Type: IC

The work shown here evaluates the HPAE-PAD assay method described in the USP monographs for kanamycin and amikacin8,9 and describes a revised HPAE-PAD method using a Dionex CarboPac™ MA1 column and disposable Au-on-polytetrafluoroethylene (PTFE) working electrodes for the analysis of kanamycin and amikacin. The described method provides good sensitivity, high sample throughput, and retention time reproducibility for kanamycin and amikacin.