AN1149: Profiling Fructosyloligosaccharides (FOS)-containing samples by HPAE-PAD

Instrument Type: IC

In this work, the oligo- and polysaccharide distribution of three different inulin samples has been characterized by HPAE-PAD. In addition, HPAE-PAD profiling was demonstrated as a method to determine FOS content in prebiotic samples and variation in prebiotics on addition of probiotics. We used Inulin-FOS and chicory root inulin as prebiotic samples. For probiotics, we used Yakult drink, a well known probiotic dairy product. In this method, separation of individual oligosaccharides in FOS was achieved on a Dionex CarboPac PA200 column. An ICS-6000 can be used for this application.

AN83: Size-Exclusion Chromatography of Polysaccharides with Pulsed Amperometric Detection (PAD).

Instrument Type: IC

Size-exclusion chromatography is used in carbohydrate research to determine molecular weight distributions of polysaccharides. Size-exclusion columns separate biological macromolecules by hydrodynamic size. Pulsed amperometric detection (PAD), provides sensitive and specific detection of carbohydrates. Here, fractionated pullulan standards were used to determine the separation range of linear carbohydrates.This application note shows how SEC can be coupled to PAD.

AU150: Determination of Plant-Derived Neutral Oligo- and Polysaccharides

Instrument Type: IC

Since the publication of Application Note 67, Dionex introduced the Dionex CarboPac™ PA200, a column designed for even higher-resolution separations than the Dionex CarboPac PA1 or PA100 columns. The improved resolution is due in part to the PA200’s significantly smaller substrate (5.5 μm) and latex (43 nm) beads. These and other changes produce a column that has greater resolution and requires a lower sodium acetate concentration to elute a given oligosaccharide than the PA100. The AU highlights the benefits of the PA200 for these applications.

AN67: Determination of Plant-Derived Neutral Oligo- and Polysaccharides

Instrument Type: IC

This application note describes how to develop an HPAE-PAD method for separating plant-derived neutral oligo- and polysaccharides. Amylopectin and fructan separations demonstrate column, eluent, and gradient choices for developing rugged HPAE-PAD methods for neutral oligo- and polysaccharides.