There is increasing demand to provide rapid and selective screening techniques for a wide range of antibiotic drugs. Their abuse as a feed supplement in food animals is of growing concern and is subject to regulations globally. Creating screening methods for multiple analytes is more cost effective than dedicated methods for fewer analytes. This application shows the advantages of using the Thermo Scientific Accucore Vanquish C18 UHPLC 1.5 µm column and Vanquish UHPLC system for the analysis of thirty-six antibiotics.

Sulfonamides are used widely as anti-inflammatory medications for humans and livestock, and have recently become compounds of interest to regulatory agencies worldwide. Triple stage quadrupole LC-MS systems are often used for highly sensitive quantitative analyses of environmental pollutants. Performance of the LC-MS and sample pre-treatment are 2 critical factors that determine efficiency and accuracy in detection of low concentrations of pollutants in environmental samples. We describe a method for the quantitative analysis of sulfonamide antibiotics using EQuan.

The Thermo Scientific HPLC system is applied for the analysis of sulfa drugs. The separation is performed using a Thermo Scientific Acclaim PolarAdvantage column, with UV detection at 254 nm.

The Thermo Scientific UltiMate 3000 RS HPLC system is applied for the determination of sulfonamide antibiotics. The separation is performed on a Thermo Scientific Acclaim 120 C18 RSLC column, with UV detection at 265 nm.

The Thermo Scientific HPLC system is applied for the determination of sulfonamide antibiotic residues in milk. The separation is performed on a Thermo Scientific Acclaim 120 C18 HPLC column, with UV detection at 265 nm.