Fast, high resolution size exclusion separation of a protein mix by bio-compatible UHPLC

Instrument Type: HPLC (Biocompatible)

A mix of five proteins in varying size was separated on a Thermo Scientific MabPac SEC-1 column using a Thermo Scientific Vanquish UHPLC system. The molecular weight range was 0.13 kDa – 670 kDa. Elution with high resolution of all five proteins was achieved within 15 minutes.

Simple gradient method for the analysis of protein standards by micro LC

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the analysis of protein standards. The separation is performed on a Thermo Scientific Acclaim 300 C18 micro LC column, with UV detection at 214 nm.

Rapid separation of high and low molecular weight peptides using a solid core C18 HPLC column

Instrument Type: HPLC

The separation of peptides was successfully achieved on the Thermo Scientific Accucore C18 HPLC column. Basline resolution was achieved between high and low molecular weight peptides with a simple gradient method with a run time of 10 minutes

Monitoring protein deamidation using a Thermo Scientific ProPac WCX-10 column

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific UltiMate 3000 Titanium HPLC system is applied to monitor the products of protein deamidation. The separation is performed on a Thermo Scientific ProPac WCX-10 column, with UV detection at 280 nm.