The accurate measurement of the amount of sugar in final molasses is economically important to sugar mills, because the purchase price of the final molasses is directly related to its sugar content. A high-performance anion exchange method with pulsed amperometric detection (HPAE-PAD) was developed to accurately determine the sugar concentrations of cane and beet final molasses samples.

Fruit juice adulteration presents an economic & regulatory problem. Investigators using high performance anion exchange chromatography with pulsed amperometric detection(HPAE-PAD) discovered several components in beet medium invert sugar(BMIS) that are not present in orange juice. The selectivity of anion-exchange chromatography, especially for oligosaccharides, the sensitivity and specificity of pulsed amperometric detection make HPAE-PAD uniquely suited to this analysis. Today we would use a different electrochemical waveform (see TN21) and post-column addition of NaOH would not be required.

Fruit juice adulteration presents an economic & regulatory problem. Investigators using high performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) have discovered several components in beet medium invert sugar (BMIS) that are not present in orange juice. The selectivity of anion-exchange chromatography, for oligosaccharides, and the sensitivity and specificity of pulsed amperometric detection make HPAE-PAD uniquely suited to this analysis. Today we would use a different electrochemical waveform (see TN21) and post-column addition of NaOH would not be required.

This Application Note describes the use of two different anion-exchange columns with amperometric detection to analyze simple sugars, sugar alcohols, alcohols, and glycols in yeast and bacterial fermentation broths. The yeast Saccharomyces cerevisiae in Yeast Extract-Peptone-Dextrose (YPD) broth and the bacteria Escherichia coli (E. coli) in Luria-Bertani (LB) broth are common eukaryotic and prokaryotic fermentation systems, respectively. This record shows a method that is best for mono- and disaccharides.

Fruit juice adulteration presents an economic & regulatory problem. Investigators using high performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) discovered several components in beet medium invert sugar(BMIS) that are not present in orange juice. The selectivity of anion-exchange chromatography, especially for oligosaccharides, the sensitivity and specificity of pulsed amperometric detection make HPAE-PAD uniquely suited to this analysis. Today we would use a different electrochemical waveform (see TN21) and post-column addition of NaOH would not be required