The Thermo Scientific HPLC system is applied for the analysis of basic drugs. The separation is performed on a Thermo Scientific Acclaim 120 C18 column, with UV detection at 220 nm. This application is used as a routine lot qualification test for the Acclaim 120 C18 column stationary phase. Low silanol activity preserves the good peak shape for amitriptyline as the sample concentration decreases (amitriptyline mass for trace A is 1200 ng, for trace B is 400 ng, and for trace C 94 ng).

The Thermo Scientific HPLC system is applied for the analysis of beta-blockers. The separation is performed using a Thermo Scientific Acclaim 120 C8 column, with UV detection at 214 nm.

The Thermo Scientific HPLC system is applied for the separation of basic compounds. The separation was performed using a Thermo Scientific Accucore RP-MS column in under 3 minutes, with UV detection at 215nm.

The Thermo Scientific HPLC system is applied for the determination of six beta blockers. The separation is performed on a Thermo Scientific Accucore RP-MS HPLC column, with UV detection at 220 nm.

Thermo Scientific HyperSep solid-supported liquid-liquid extraction (SLE) is compared to traditional liquid-liquid extraction (LLE) for the extraction of beta blockers. The analysis is performed on a Thermo Scientific HPLC system using a Thermo Scientific Accucore C18 HPLC column. By using Thermo Scientific HyperSep SLE instead of traditional LLE methods, recovery of the analytes can be improved and the extraction time significantly reduced. For Research Use Only. Not for use in diagnostic procedures.