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Displaying 1-5 of 8 results for Tag: Ovalbumin

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Fast, high resolution size exclusion separation of a protein mix by bio-compatible UHPLC

Instrument Type: HPLC (Biocompatible)

A mix of five proteins in varying size was separated on a Thermo Scientific MabPac SEC-1 column using a Thermo Scientific Vanquish UHPLC system. The molecular weight range was 0.13 kDa – 670 kDa. Elution with high resolution of all five proteins was achieved within 15 minutes.

Improved analysis of four peptide maps on a Thermo Scientific Acclaim 120 C18 HPLC column

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 RSLC system is applied for the analysis of peptide mapping. The separation of four peptide maps was achieved on a Thermo Scientific Acclaim 120 C18 column in 50 minutes with UV detection.

Increased resolution of tryptic digests of proteins using a Thermo Scientific Acclaim RSLC C18 HPLC column

Instrument Type: UHPLC

In this work, a digestion of bovine serum albumin is resolved using the Thermo Scientific Acclaim RSLC C18 into 127 peaks with an average peak width of 0.27 minutes. For a 90 minute gradient, this works out to a theoretical peak capacity of >300.

Improved analysis of intact proteins using a Thermo Scientific Accucore 150-C4 150 Å pore diameter nanoLC column

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the analysis of intact proteins (*indicates an impurity from carbonic anhydrase). The separation is performed on a Thermo Scientific Accucore 150-C4 150 Å pore diameter nanoLC column, with UV detection at 214 nm. The 150 Å pore size enables the effective analysis of molecules unable to penetrate into smaller diameter pores, whilst the low hydrophobicity C4 phase results in protein separation by hydrophobicity.

Improved analysis of intact proteins using a Thermo Scientific Accucore 150-C4 150 Å pore diameter HPLC column

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the analysis of intact proteins. The separation is performed on a Thermo Scientific Accucore 150-C4 150 Å pore diameter HPLC column in under 10 minutes, with UV detection at 214 nm. The 150 Å pore size enables the effective analysis of molecules unable to penetrate into smaller diameter pores, whilst the low hydrophobicity C4 phase results in protein separation by hydrophobicity.