The Thermo Scientific Acclaim Trinity P2 column was used to determine a small amount of guanidine in a background of phosphate buffered saline (PBS). Even though the high concentration of sodium chloride overloads the column, it has sufficient resolution for quantifying all the components.

The Thermo Scientific UltiMate 3000 HPLC system is applied for the analysis of the API (active pharmaceutical ingredient) amphetamine and its counterions. The separation is performed using a Thermo Scientific Acclaim Trinity P1 column, with both UV and charged aerosol detection. Amphetamine and saccharin respond to UV detection; aspartate, saccharin and sulfate respond to charged-aerosol detection. Note that metal contamination will cause peak tailing, and before use, the column should be cleaned with a chelating agent at pH 4.

The Thermo Scientific UltiMate 3000 HPLC system is applied for the analysis of metaraminol bitartrate. The separation is performed on a Thermo Scientific Acclaim Trinity P1 HPLC column, with UV detection at 210 nm.

The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of phenylephrine HCl. The separation is performed using a Thermo Scientific Acclaim Trinity P1 column, with charged aerosol detection. The Thermo Scientific Acclaim Trinity P1 is a unique column that provides reversed-phase, weak anion-exchange, and strong cation-exchange retention mechanisms at the same time, thus providing an ideal solution for simultaneous separation of the drug molecule and its counterion.

The Thermo Scientific HPLC system is applied for the analysis of Melamine in powdered milk. The sample was prepared using HyperSep Retain CX solid phase extraction (SPE) cartridges, and the separation performed on a Thermo Scientific Accucore HILIC HPLC column in less than 2 minutes, with MS/MS detection.