Fruit juice adulteration presents an economic & regulatory problem. Investigators using high performance anion exchange chromatography with pulsed amperometric detection(HPAE-PAD) discovered several components in beet medium invert sugar(BMIS) that are not present in orange juice. The selectivity of anion-exchange chromatography, especially for oligosaccharides, the sensitivity and specificity of pulsed amperometric detection make HPAE-PAD uniquely suited to this analysis. Today we would use a different electrochemical waveform (see TN21) and post-column addition of NaOH would not be required.

Fruit juice adulteration presents an economic & regulatory problem. Investigators using high performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) have discovered several components in beet medium invert sugar (BMIS) that are not present in orange juice. The selectivity of anion-exchange chromatography, for oligosaccharides, and the sensitivity and specificity of pulsed amperometric detection make HPAE-PAD uniquely suited to this analysis. Today we would use a different electrochemical waveform (see TN21) and post-column addition of NaOH would not be required.

Fruit juice adulteration presents an economic & regulatory problem. Investigators using high performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) discovered several components in beet medium invert sugar(BMIS) that are not present in orange juice. The selectivity of anion-exchange chromatography, especially for oligosaccharides, the sensitivity and specificity of pulsed amperometric detection make HPAE-PAD uniquely suited to this analysis. Today we would use a different electrochemical waveform (see TN21) and post-column addition of NaOH would not be required

Determinations of organic acid profiles in fruit juices are used in the beverage industry to characterize flavor, identify spoilage and potential sources of adulteration, and to meet labeling requirements for food products. In this study, inorganic anions and organic acids from a diluted orange juice sample are separated by anion-exchange chromatography on a capillary size IonSwift MAX-100 monolith IC column and detected by suppressed conductivity detection. For more information on organic acid determinations in fruit juices, please review AN143. An ICS-6000 can be used for this application.

The Thermo Scientific HPLC system is applied for the analysis of organic acids in orange juice. The separation is performed on a Thermo Scientific Acclaim Organic Acid (OA) column, with UV detection at 210 nm. The samples were prepared using Thermo Scientific Dionex OnGuard II P SPE cartridges.