To describe a fast and reproducible optimization procedure for the isolation of opioids from human urine utilizing solid phase extraction (SPE). Subsequent analysis was carried out with liquid chromatography separation coupled to triple quadrupole mass spectrometry detection (LC-MS/MS).

Forensic toxicologists need an economical solution to screen for a virtually unlimited number of compounds in urine. Here we present a method for screening using a dilute-and-shoot approach with the Q Exactive Focus mass spectrometer and Thermo Scientific ToxFinder software. For forensic use only.

Due to its increased abuse, forensic and testing labs test routinely for the presence of buprenorphine and its metabolite norbuprenorphine. To reduce their case backlog, these labs need cost-efficient analytical tests on hundreds of samples. We developed a high-throughput screening and quantitation LC-MS/MS method in which urine samples are quickly prepared and analyzed by a short chromatographic method implemented on a four-channel LC-MS system for improved productivity. For forensic use only.

Forensic toxicologists need an economical instrument capable of both screening a large number of compounds and quantifying smaller panels to industry-established limits. Here we present a method for quantitation of six opiates - morphine, codeine, hydromorphone, hydrocodone, oxymorphone, and oxycodone - in human urine down to low ng/mL levels. This work was performed on a Q Exactive Focus hybrid quadrupole Orbitrap mass spectrometer. For forensic use only.

Forensic toxicologists face an ever-expanding list of compounds for analysis. This note presents work done using a next-generation triple quadrupole mass spectrometer with fast SRM acquisition speed for quantitation of 122 analytes in a single chromatographic run, where scan speed does not impact sensitivity or quantitative performance. Compounds analyzed include opiates, opioids, benzodiazepines, barbiturates, amphetamines, tricyclic antidepressants, illicit compounds, and more. For forensic use only.