Metformin (N,N-dimethylimidodicarbonimidic diamide) is a widely prescribed orally administered antidiabetic drug for the treatment of type 2 diabetes. This application presents a IC method used for quantitation of dimethylamine in metformin hydrochloride. This method uses a Thermo Scientific Dionex IonPac CS19 cation-exchange analytical column with methanesulfonic acid eluent produced by an eluent generator. The method separates dimethylamine from other cations typically present in drug products.

The Thermo Scientific Dionex UltiMate 3000 system is applied for the determination of metformin. The separation is performed on a Thermo Scientific Syncronis HILIC 5 µm column, with UV detection at 225 nm and a total run time of 6 minutes.

This work demonstrates the determination of metformin (API) and its counterion (chloride) using a simple isocratic method. Thermo Scientific Acclaim Trinity P2 columns offer cation-exchange, anion-exchange and HILIC retention mechanisms on the same phase, thus they are ideal for separating both cationic and anionic species simultaneously.

The Thermo Scientific HPLC system is applied for the determination of melamine and metformin hydrochloride. The separation is performed on a Thermo Scientific BioBasic SCX HPLC column, with UV detection at 218 nm.

The Thermo Scientific HPLC system is applied for the determination of metformin and voglibose. The separation is performed on a Thermo Scientific Accucore HILIC HPLC column, with MS detection.