Honey is a complex, solution of saccharides with low water content resulting from the action of the enzyme invertase on sucrose, which creates other dissaccharides and polysaccharides. Other components include the enzymes themselves, organic acids, and minerals, all of which contribute to the flavors and preservative properties of this energy source. A UHPLC-CAD method uses HILIC to separate over 30 components of honey in 45 minutes.

Many biopharmaceutical formulations use carbohydrates to enhance the stability of proteins. This method is an example of a "heart-cut" 2D HPLC method, separating the carbohydrate analyte from the protein. The method uses two charge aerosol detectors, as an example, but one can be substituted by UV. Calibration for the Maltose is from 15.6 - 2000 ng o.c. If a second Corona Veo SD is used for the Protein, it uses the parameters described as "Veo SD".

In many of the vaccines and other formulations that are used in the prevention and treatment of many conditions and illnesses contain carbohydrates, as a part of their formulation. Six carbohydrates were identified as important components in biotechnological formulations: sorbitol, mannitol, sucrose, lactose, maltose, and trehalose. These are separated by HILIC-HPLC and detected by the universal Corona charged aerosol detector.

This application note describes the use of ion-exchange or ion-exclusion chromatography for the determination of five classes of compounds of interest to the brewing industry, including: carbohydrates, alcohols, organic acids, inorganic anions, and inorganic cations. One of two forms of electrochemical detection is used, pulsed amperometry or conductivity detection.This part of the application note describes the malto-oligosaccharide method which uses pulsed amperometry detection. This was formerly Dionex AN46.

The separation of five sugars was performed using the Thermo Scientific Hypersil GOLD Amino column in HILIC mode with RI detection.