Improved separation of closely related glucocorticoids with reduced method complexity High-throughput analysis possible through a reduced complexity, rapid, two-minute isocratic method Associated reduction in cost per sample through reduced mobile phase consumption and waste generation

The Thermo Scientific HPLC system is applied for the analysis of hydrocortisone in skin ointment. The separation is performed on a Thermo Scientific Acclaim 120 C8 column, with UV detection at 245 nm.

The Thermo Scientific Hypersil GOLD Silica column was used to separate seven steroids using a normal-phase HPLC method with UV detection at 254 nm.

The Thermo Scientific UltiMate 3000 HPLC system is applied for the analysis of hydrocortisone in topical cream. The separation is performed using a Thermo Scientific Acclaim 120 C18 column, with UV detection at 245 nm.

A fast and reliable separation for quality control of small molecule drug candidates is demonstrated using Thermo Scientific Accucore aQ HPLC columns. The method run time was 3.25 minutes with each compound adequately resolved. The excellent peak shape was maintained for more than 11000 injections.