We demonstrated a simple workflow for the simultaneous analysis of melamine and analogues in pharmaceutical components using a Q Exactive benchtop Orbitrap mass spectrometer. The sensitive, simple and robust method employed the Thermo Fisher Scientific Q Exactive high resolution Orbitrap mass spectrometry coupled with UHPLC and HILIC mode chromatographic separation. Simultaneous detection and quantification of melamine and its analogues in Albumin and Guar gum were achieved with a high degree of confidence.

The Thermo Scientific HPLC system is applied for the analysis of retinyl palmitate and retinyl acetate. The separation is performed on a Thermo Scientific Acclaim HILIC-10 column, with UV detection at 285 nm.

The Thermo Scientific HPLC system is applied for the analysis of urea. The separation is performed on a Thermo Scientific Hypersil GOLD HILIC column in less than 8 minutes, with MS detection.

The Thermo Scientific HPLC system is applied for the analysis of vitamin K1 and K2. The separation is performed on a Thermo Scientific Acclaim HILIC-10 column, with UV detection at 265 nm.

The Thermo Scientific Dionex UltiMate 3000 system is applied for the determination of metformin. The separation is performed on a Thermo Scientific Syncronis HILIC 5 µm column, with UV detection at 225 nm and a total run time of 6 minutes.