The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the separation of multivitamins (Folic Acid,Vitamins C, B12, and E from a Multivitamin Capsule. The separation was performed on a Thermo Scientific Acclaim Polar Advantage-II HPLC column with a UV detection at 270 nm. With a run time of 35 minutes, the method allows easy separation of Vitamin C, B12, Folic Acid and E from Multivitamin Capsule.

Vitamin K1 isomers and related compounds are separated using a silica HPLC column and a normal phase solvent system. The correct silica type and mobile phase equilibration are important considerations in order to achieve good resolution and retention time reproducibility.

The Thermo Scientific HPLC system is applied for the analysis of vitamin K1 and K2. The separation is performed on a Thermo Scientific Acclaim HILIC-10 column, with UV detection at 265 nm.

The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of fat-soluble vitamins. The separation is performed using a Thermo Scientific Acclaim Phenyl-1 column, with UV detection at 220 nm.

The Thermo Scientific HPLC system is applied for the analysis of fat-soluble vitamins and carotenoids in a vitamin tablet. The separation is performed using a Thermo Scientific Acclaim PolarAdvantage (PA) column, with UV detection at 450 and 285 nm.